Rakuten Aspyrian is searching for a highly motivated individual to support cell culture maintenance, scale-up, and engineering new cell lines for Aspyrian’s Translational Research team. In addition to these primarily responsibilities, this candidate will also assist with executing in vitro assays to support the company’s drug development programs.
Key Duties and Responsibilities:
• Key responsibilities include maintenance and coordinated scale-up of murine and human cancer cell lines for in vitro assays and in vivo implants, as well as engineering and characterizing new stable clonal cell lines for this purpose.
• Participate in team meetings to provide input into the selection, purchase, and implementation of appropriate cell lines used to support program goals.
• Responsible for troubleshooting of all cell culture related equipment to support research activities and coordinate with lab management to handle all maintenance and repairs.
• Responsible for the preparation of buffers, media and other required reagents as need to support cell culture activities.
• Responsible for maintaining raw materials and lab supplies, and oversight of inventory of all required reagents in coordination with lab management.
• Oversee on-site and off-site cell bank generation and database documentation.
• Participate and provide input into the optimization of workflows related to cell culture activities.
• Participate in the identification and evaluation of new equipment and technology related to cell culture activities.
• Author guidelines, data sheets, and SOPs related to research cell culture activities.
• Support in vitro, in vivo, and ex vivo cell culture related activities as needed.
• Present data and summaries at weekly team meetings, ensuring effective communication of ideas and results, and proactively identify and suggest solutions in a multidisciplinary environment.
• Other duties as assigned.
Desired Education, Skills, and Experience:
• BS/MS with 5+ years of experience using mammalian cell culture techniques in a pharmaceutical research and development environment or a related discipline.
• In-depth experience working with a variety of primary, immortalized, and engineered cell lines is required.
• Experience working with and manipulating human and murine immune cell lines is highly desirable, especially for in vivo tumor implantation (allograft and xenograft).
• Thorough understanding of BSL-1 and BSL-2 safety practices is required.
• Strong attention to detail, organizational skills, innovative thinking, and proactive problem-solving skills are required.
• Candidate must be able to effectively communicate and present ideas and results in a cross-functional team environment.
Location: San Diego, CA
Department: Translational Research
Min. Experience: Associate